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dmem high glucose hepes  (Thermo Fisher)


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    Thermo Fisher dmem high glucose hepes
    Dmem High Glucose Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem+high+glucose+hepes/HEPES/pm41985787-166-55-60
    Average 99 stars, based on 1 article reviews
    dmem high glucose hepes - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: ACE2-EGFR-MAPK signaling contributes to SARS-CoV-2 infection.
    Article Snippet: .. Caco-2 cells were cultured in DMEM (Gibco) High Glucose (4.5 mg/l) HEPES and supplemented with 10% FBS (Gibco) and 1% penicillin– streptomycin (Invitrogen) at 37°C in a 5% CO2 atmosphere. .. The following concentrations were used throughout the experiments under serum-starved conditions: 100 ng/ml Spike-RBD (original strain—Histagged Spike-RBD Protein; InvivoGen), 10 ng/ml EGF (ImmunoTools), 5 μM EK1, 10 μg/ml bamlanivimab (LY-CoV555), 10 μg/ml casirivimab (REGN10933), 10 μg/ml imdevimab (REGN10987), 10 μM MEK1/2inhibitor (U0126), 5 μM EGFR inhibitor (erlotinib).

    Article Title: ACE2-EGFR-MAPK signaling contributes to SARS-CoV-2 infection
    Article Snippet: .. Caco-2 cells were cultured in DMEM (Gibco) High Glucose (4.5 mg/l) HEPES and supplemented with 10% FBS (Gibco) and 1% penicillin–streptomycin (Invitrogen) at 37°C in a 5% CO 2 atmosphere. .. The following concentrations were used throughout the experiments under serum-starved conditions: 100 ng/ml Spike-RBD (original strain—His-tagged Spike-RBD Protein; InvivoGen), 10 ng/ml EGF (ImmunoTools), 5 μM EK1, 10 μg/ml bamlanivimab (LY-CoV555), 10 μg/ml casirivimab (REGN10933), 10 μg/ml imdevimab (REGN10987), 10 μM MEK1/2-inhibitor (U0126), 5 μM EGFR inhibitor (erlotinib).

    Polymerase Chain Reaction:

    Article Title: A dileucine motif in TMEM163 is essential for its binding with both AP-3 and BLOC-1 complex.
    Article Snippet: Additional antibodies included: rabbit anti-Myc pAb (C3956, SigmaAldrich), mouse anti-β-actin mAb (A5441, Sigma-Aldrich), mouse anti-Flag mAb (F7425, Sigma-Aldrich), rabbit anti-Rab7 mAb (9367, Cell Signaling Technology), rabbit anti-AP3B1 pAb (13384-1-AP, Proteintech), mouse anti-EEA1 mAb (610457, BD Pharmingen), rabbit anti-GST pAb (Bs-2122R, Bioss) and HRP-conjugated mouse anti-rabbit IgG (BE0107, Easybio). .. Other reagents used in this study are: TPA (12-O-tetradecanoylphorbol-13-acetate; #4174, Cell Signaling Technology), mepacrine (Q2876, Sigma-Aldrich), CellMask Deep Red (C10046, Thermo Fisher Scientific), KOD One PCR Master Mix (KMM-101, TOYOBO), Anti-FLAG agarose beads (M8823, Sigma-Aldrich), GST-Sep lutathione MagBeads (20562ES, Yeasen), JetOptimus transfection reagent (10100006, Polyplus), Lipofectamine 2000 (11668-019, Invitrogen), HyClone RPMI 1640 media (SH30809.01, HyClone), DMEM High Glucose HEPES (12430054, Gibco), FBS (10099-141, Gibco), Protease inhibitor cocktail (P8340, Sigma-Aldrich), RNeasy Mini Kit (74104, QIAGEN), iScript cDNA Synthesis Kit (1708891, BIO-RAD), SuperReal Fluorescence Quantitative Premix Plus (SYBR Green) Kit (FP205-02, TIANGEN), RNAiMAX (13778150, Jo urn al Pr e-p roo f 14 Thermo Fisher Scientific) and FluoZin-3 (F24195, Life Technologies). ..

    Transfection:

    Article Title: A dileucine motif in TMEM163 is essential for its binding with both AP-3 and BLOC-1 complex.
    Article Snippet: Additional antibodies included: rabbit anti-Myc pAb (C3956, SigmaAldrich), mouse anti-β-actin mAb (A5441, Sigma-Aldrich), mouse anti-Flag mAb (F7425, Sigma-Aldrich), rabbit anti-Rab7 mAb (9367, Cell Signaling Technology), rabbit anti-AP3B1 pAb (13384-1-AP, Proteintech), mouse anti-EEA1 mAb (610457, BD Pharmingen), rabbit anti-GST pAb (Bs-2122R, Bioss) and HRP-conjugated mouse anti-rabbit IgG (BE0107, Easybio). .. Other reagents used in this study are: TPA (12-O-tetradecanoylphorbol-13-acetate; #4174, Cell Signaling Technology), mepacrine (Q2876, Sigma-Aldrich), CellMask Deep Red (C10046, Thermo Fisher Scientific), KOD One PCR Master Mix (KMM-101, TOYOBO), Anti-FLAG agarose beads (M8823, Sigma-Aldrich), GST-Sep lutathione MagBeads (20562ES, Yeasen), JetOptimus transfection reagent (10100006, Polyplus), Lipofectamine 2000 (11668-019, Invitrogen), HyClone RPMI 1640 media (SH30809.01, HyClone), DMEM High Glucose HEPES (12430054, Gibco), FBS (10099-141, Gibco), Protease inhibitor cocktail (P8340, Sigma-Aldrich), RNeasy Mini Kit (74104, QIAGEN), iScript cDNA Synthesis Kit (1708891, BIO-RAD), SuperReal Fluorescence Quantitative Premix Plus (SYBR Green) Kit (FP205-02, TIANGEN), RNAiMAX (13778150, Jo urn al Pr e-p roo f 14 Thermo Fisher Scientific) and FluoZin-3 (F24195, Life Technologies). ..

    Protease Inhibitor:

    Article Title: A dileucine motif in TMEM163 is essential for its binding with both AP-3 and BLOC-1 complex.
    Article Snippet: Additional antibodies included: rabbit anti-Myc pAb (C3956, SigmaAldrich), mouse anti-β-actin mAb (A5441, Sigma-Aldrich), mouse anti-Flag mAb (F7425, Sigma-Aldrich), rabbit anti-Rab7 mAb (9367, Cell Signaling Technology), rabbit anti-AP3B1 pAb (13384-1-AP, Proteintech), mouse anti-EEA1 mAb (610457, BD Pharmingen), rabbit anti-GST pAb (Bs-2122R, Bioss) and HRP-conjugated mouse anti-rabbit IgG (BE0107, Easybio). .. Other reagents used in this study are: TPA (12-O-tetradecanoylphorbol-13-acetate; #4174, Cell Signaling Technology), mepacrine (Q2876, Sigma-Aldrich), CellMask Deep Red (C10046, Thermo Fisher Scientific), KOD One PCR Master Mix (KMM-101, TOYOBO), Anti-FLAG agarose beads (M8823, Sigma-Aldrich), GST-Sep lutathione MagBeads (20562ES, Yeasen), JetOptimus transfection reagent (10100006, Polyplus), Lipofectamine 2000 (11668-019, Invitrogen), HyClone RPMI 1640 media (SH30809.01, HyClone), DMEM High Glucose HEPES (12430054, Gibco), FBS (10099-141, Gibco), Protease inhibitor cocktail (P8340, Sigma-Aldrich), RNeasy Mini Kit (74104, QIAGEN), iScript cDNA Synthesis Kit (1708891, BIO-RAD), SuperReal Fluorescence Quantitative Premix Plus (SYBR Green) Kit (FP205-02, TIANGEN), RNAiMAX (13778150, Jo urn al Pr e-p roo f 14 Thermo Fisher Scientific) and FluoZin-3 (F24195, Life Technologies). ..

    cDNA Synthesis:

    Article Title: A dileucine motif in TMEM163 is essential for its binding with both AP-3 and BLOC-1 complex.
    Article Snippet: Additional antibodies included: rabbit anti-Myc pAb (C3956, SigmaAldrich), mouse anti-β-actin mAb (A5441, Sigma-Aldrich), mouse anti-Flag mAb (F7425, Sigma-Aldrich), rabbit anti-Rab7 mAb (9367, Cell Signaling Technology), rabbit anti-AP3B1 pAb (13384-1-AP, Proteintech), mouse anti-EEA1 mAb (610457, BD Pharmingen), rabbit anti-GST pAb (Bs-2122R, Bioss) and HRP-conjugated mouse anti-rabbit IgG (BE0107, Easybio). .. Other reagents used in this study are: TPA (12-O-tetradecanoylphorbol-13-acetate; #4174, Cell Signaling Technology), mepacrine (Q2876, Sigma-Aldrich), CellMask Deep Red (C10046, Thermo Fisher Scientific), KOD One PCR Master Mix (KMM-101, TOYOBO), Anti-FLAG agarose beads (M8823, Sigma-Aldrich), GST-Sep lutathione MagBeads (20562ES, Yeasen), JetOptimus transfection reagent (10100006, Polyplus), Lipofectamine 2000 (11668-019, Invitrogen), HyClone RPMI 1640 media (SH30809.01, HyClone), DMEM High Glucose HEPES (12430054, Gibco), FBS (10099-141, Gibco), Protease inhibitor cocktail (P8340, Sigma-Aldrich), RNeasy Mini Kit (74104, QIAGEN), iScript cDNA Synthesis Kit (1708891, BIO-RAD), SuperReal Fluorescence Quantitative Premix Plus (SYBR Green) Kit (FP205-02, TIANGEN), RNAiMAX (13778150, Jo urn al Pr e-p roo f 14 Thermo Fisher Scientific) and FluoZin-3 (F24195, Life Technologies). ..

    Fluorescence:

    Article Title: A dileucine motif in TMEM163 is essential for its binding with both AP-3 and BLOC-1 complex.
    Article Snippet: Additional antibodies included: rabbit anti-Myc pAb (C3956, SigmaAldrich), mouse anti-β-actin mAb (A5441, Sigma-Aldrich), mouse anti-Flag mAb (F7425, Sigma-Aldrich), rabbit anti-Rab7 mAb (9367, Cell Signaling Technology), rabbit anti-AP3B1 pAb (13384-1-AP, Proteintech), mouse anti-EEA1 mAb (610457, BD Pharmingen), rabbit anti-GST pAb (Bs-2122R, Bioss) and HRP-conjugated mouse anti-rabbit IgG (BE0107, Easybio). .. Other reagents used in this study are: TPA (12-O-tetradecanoylphorbol-13-acetate; #4174, Cell Signaling Technology), mepacrine (Q2876, Sigma-Aldrich), CellMask Deep Red (C10046, Thermo Fisher Scientific), KOD One PCR Master Mix (KMM-101, TOYOBO), Anti-FLAG agarose beads (M8823, Sigma-Aldrich), GST-Sep lutathione MagBeads (20562ES, Yeasen), JetOptimus transfection reagent (10100006, Polyplus), Lipofectamine 2000 (11668-019, Invitrogen), HyClone RPMI 1640 media (SH30809.01, HyClone), DMEM High Glucose HEPES (12430054, Gibco), FBS (10099-141, Gibco), Protease inhibitor cocktail (P8340, Sigma-Aldrich), RNeasy Mini Kit (74104, QIAGEN), iScript cDNA Synthesis Kit (1708891, BIO-RAD), SuperReal Fluorescence Quantitative Premix Plus (SYBR Green) Kit (FP205-02, TIANGEN), RNAiMAX (13778150, Jo urn al Pr e-p roo f 14 Thermo Fisher Scientific) and FluoZin-3 (F24195, Life Technologies). ..

    SYBR Green Assay:

    Article Title: A dileucine motif in TMEM163 is essential for its binding with both AP-3 and BLOC-1 complex.
    Article Snippet: Additional antibodies included: rabbit anti-Myc pAb (C3956, SigmaAldrich), mouse anti-β-actin mAb (A5441, Sigma-Aldrich), mouse anti-Flag mAb (F7425, Sigma-Aldrich), rabbit anti-Rab7 mAb (9367, Cell Signaling Technology), rabbit anti-AP3B1 pAb (13384-1-AP, Proteintech), mouse anti-EEA1 mAb (610457, BD Pharmingen), rabbit anti-GST pAb (Bs-2122R, Bioss) and HRP-conjugated mouse anti-rabbit IgG (BE0107, Easybio). .. Other reagents used in this study are: TPA (12-O-tetradecanoylphorbol-13-acetate; #4174, Cell Signaling Technology), mepacrine (Q2876, Sigma-Aldrich), CellMask Deep Red (C10046, Thermo Fisher Scientific), KOD One PCR Master Mix (KMM-101, TOYOBO), Anti-FLAG agarose beads (M8823, Sigma-Aldrich), GST-Sep lutathione MagBeads (20562ES, Yeasen), JetOptimus transfection reagent (10100006, Polyplus), Lipofectamine 2000 (11668-019, Invitrogen), HyClone RPMI 1640 media (SH30809.01, HyClone), DMEM High Glucose HEPES (12430054, Gibco), FBS (10099-141, Gibco), Protease inhibitor cocktail (P8340, Sigma-Aldrich), RNeasy Mini Kit (74104, QIAGEN), iScript cDNA Synthesis Kit (1708891, BIO-RAD), SuperReal Fluorescence Quantitative Premix Plus (SYBR Green) Kit (FP205-02, TIANGEN), RNAiMAX (13778150, Jo urn al Pr e-p roo f 14 Thermo Fisher Scientific) and FluoZin-3 (F24195, Life Technologies). ..



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